ZEISS Axiovert 5 digital mono CI – Cell Culture Microscope

Artificial Intelligence in the Cell Culture Lab

– AI-powered image recognition for cell counting and cell confluence
– Automated analysis of cell cultures with trained AI models
– Workflow: Image acquisition, cell nucleus counting, calculation of area coverage
– Report with image, cell count, and percentage of area coverage in a few seconds
– Ideal for multi-user environments and standardized workflows

Documentation at the push of a button

– Release button on the stand for fast image acquisition
– Single image acquisition or multi-channel fluorescence (up to 4 fluorescence channels + 1 transmitted light channel)
– AI workflow for cell counting and cell confluence: acquire image, let it analyze automatically
– Video recording for documenting dynamic processes
– Intuitive operating concept for routine, education, and research

Axiovert 5 digital microscope

– Complete Axiovert 5 digital stand equipment with integrated mono camera
– Suitable for transmitted light brightfield, phase contrast Ph1, and fluorescence RGB-UV
– Eyepiece-less technology, prepared for tablet and Wi-Fi use
– Tablet holder for Apple iPad Pro 12.9" (Gen 4/5) and Microsoft Surface Pro 7/8
– Height and angle adjustable holder for ergonomic working

Inverted Microscope Stand

– Revolving nosepiece 6-position H M27, coded
– Reflector turret 6-position, coded for P&C modules
– Manual coarse/fine focus drive
– Focus stroke 13 mm with adjustable focus stop
– Trigger for integrated camera on the stand
– Transmitted light illumination with 10 W white light LED incl. phosphorescence block filter
– Light shield for ambient light protection
– Ergo intermediate piece H = 50 mm for optimizing viewing height

Stages and Sample Holders

– Stage 232 × 230 mm with hard anodized coating
– Mechanical stage 130 × 85 mm, mountable right/left
– Holding frame Flex M
– Inserts for:
– Microtiter plates
– Culture flasks
– Petri dishes Ø 95, 88, 65, 54, 36 mm
– Slides 76 × 26 mm
– Chambers (ibidi μ-Slide, Nunc Lab-Tek / Lab-Tek II)

Objectives

– LD A-Plan 5×/0.15 Ph1 M27
– LD A-Plan 10×/0.25 Ph1 M27
– LD A-Plan 20×/0.35 Ph1 M27
– LD A-Plan 40×/0.55 Ph1 M27

Condenser

– LD condenser 0.4
– Contrast slider for brightfield and phase contrast Ph1
– Slider 10 × 46 mm with fixed ring aperture Ph 1/0.3

Illumination – Transmitted Light & Fluorescence

– Integrated RGB-UV illumination system with 4 solid-state LEDs
– Red 630 nm: Cy5, Alexa 631, TOTO-3 and comparable dyes
– Green 565 nm: Cy3, TRITC, DsRed and comparable dyes
– Blue 470 nm: eGFP, FITC, Fluo4 and comparable dyes
– UV 385 nm: DAPI, Alexa 405, Hoechst 33258 and comparable dyes
– Average LED lifespan (wavelength dependent) typically > 60,000 h, UV > 40,000 h
– Filter set 90 HE LED with reflector module FL EC P&C

Integrated Mono Camera

– Microscopy mono camera integrated in the stand
– Resolution 2464 (H) × 2056 (V) = 5.07 megapixels
– Sensor: Sony IMX264 Exmor Pregius Global Shutter CMOS
– Pixel size 3.45 µm × 3.45 µm
– Chip size 8.5 × 7.1 mm (2/3" diagonal 11.1 mm)
– Spectral sensitivity approx. 350–1000 nm
– Max. charge per pixel approx. 10,500 e⁻
– Live image refresh rate 25 frames/s @ 1920 × 1080 pixels
– ROI readout freely definable
– 8- and 12-bit digitization
– Camera temperature stabilization to 25 °C (ambient 18–30 °C)
– External power supply 24 V DC / 5 A incl. power cable
– USB 3.0 Type-C data and 60 W fast charging cable
– USB 3.0 memory stick (Type-C & Type-A)
– Dust protection set

Labscope for iOS / Android

– Microscopy software for control, image acquisition, processing, and analysis
– Use in education, routine, and research
– Not intended for direct medical diagnostic reporting
– Extensive documentation and analysis functions
– Expandable with AI modules for cell counting and cell confluence (research)

Labscope 4.2 for Windows

– Software for controlling and acquiring images with compatible microscopes
– Supports in-vitro image analysis of physiological and pathological processes
– Applicable by professional users in life science, cytology, and pathology
– For IVD examinations: image content must match between eyepiece and monitor
– AI modules "AI Cell Confluency" and "AI Cell Counting" for research applications only

Labscope Module Multi Channel DLic

– Module for multi-channel fluorescence imaging
– Acquisition of up to 4 fluorescence channels + 1 transmitted light channel
– Smart overwriting of individual fluorescence channels
– Easy setup of fluorescence channels on manual microscopes
– Support for Smart Microscopy
– Multi-channel view with image processing
– Reporting with multi-channel images